IRESite record type: plasmid_with_promoter_and_putative_IRES_without_translational_characterization
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: 3UTR_incomplete
The mRNA/+RNA description:
Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/HIAP2(1222-1)/CAT
which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron
respectively and the part of human c-IAP1 5' UTR (nt from -1222 to nt -1 of the original sequence) mRNA cloned
in reverse complement orientation between them.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
slightly wrong.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: reverse_engineered_sequence_and_should_match_experiment_except_3UTR
The name of the promoter used to express this mRNA: CMV
Aliases of the plasmid name:
Alias: pbetaGAL/PIAH/CAT
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing part of human c-IAP1 5' UTR (nt from -1222 to nt -1 of the original sequence) inserted
between beta galactosidase and chloramphenicol acetyltransferase reporter genes in reverse complement
orientation.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: c-IAP1