IRESite record type: plasmid_with_promoter_and_putative_IRES_translationally_characterized
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: our_best_guess
The mRNA/+RNA description:
Bicistronic mRNA molecule coding for RLuc-CSFV_IRES+3nt-CAT_with_hairpin. The hairpin is placed immediately
after the KpnI site (ggtacc) which is right after the initiator ATG codon of CSFV N-pro/CAT. The construct is
is refferred in the text of the article as pRLuc-CSFV+4-hpCAT (which would mean that the first G from KpnI
site is counted as belonging to CSFV N-pro) but named in Fig. 8b as CSFV+3-hpCAT.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: end-to-end_sequence_reverse_engineered_and_should_match_experiment
The description of the protein encoded in this ORF: Chloramphenicol acetyltransferase with N-terminal 24nt hairpin stem loop coding additional 8 aminoacid
residues separated from the initiator ATG by one base on the left and by 2 bases from the CAT ORF on the
right.
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 1372-2064